Molisch’s Test

Molisch’s test is a chemical test widely used to detect the presence of carbohydrates, and it can be applied to identify glycosides, compounds containing a sugar moiety. Here is a detailed note on Molisch’s test for glycosides: Principle of Molisch’s Test Molisch’s test is based on the principle of dehydration and subsequent condensation reactions. Under … Read more

Legal’s Test – Procedure, Limitation, Application

Legal’s classic chemical test is frequently utilized in qualitatively identifying carbohydrates, including sugars present in glycosides. Glycosides comprise a sugar moiety (glycone) and a non-sugar moiety (aglycone or genin) connected through a glycosidic linkage. The test relies on the dehydration of sugars in the presence of concentrated sulfuric acid, forming furfural and hydroxymethylfurfural, which results … Read more

Identification of Glycosides

Identifying glycosides involves a series of tests and analytical techniques to determine these compounds’ presence, structure, and characteristics. Here are some common methods used for the identification of glycosides: 1. Chemical Tests Legal’s Test:Observe the reaction mixture for any color change. The development of a brown-to-black color characterizes a positive result. Molisch’s Test: A positive … Read more

Isolation of glycosides

The isolation of glycosides from natural sources involves several steps to extract, separate, and purify these compounds. Here’s a general outline of the process: 1. Selection of Source Material Choose a plant or microbial material that contains the desired glycosides based on botanical knowledge or prior research. 2. Extraction Employ suitable extraction methods to obtain … Read more

Glycosides – Occurrence and Distribution

Glycosides are organic compounds with a sugar molecule (glycone) bound to a non-sugar component (aglycone or genin) through a glycosidic linkage. The glycosidic linkage involves the sugar moiety’s attachment to the non-sugar moiety’s hydroxyl group. These compounds are widely distributed in nature and can be found in plants, microorganisms, and, to a lesser extent, in … Read more

Therapeutic activity and pharmaceutical applications of terpenoids

Due to their diverse structures and properties, terpenoids exhibit a wide range of therapeutic activities and find applications in the pharmaceutical industry. Here are some examples: 1. Anti-Inflammatory and Analgesic Properties Terpenoids:      – Many terpenoids, especially those found in essential oils (e.g., limonene, pinene), demonstrate anti-inflammatory and analgesic properties. Pharmaceutical Applications: Used in formulations … Read more

Identification of terpenoids

The identification of terpenoids involves a series of tests and analytical techniques to determine these compounds’ structural characteristics and specific properties. Here are some common identification tests for terpenoids: 1. Physical Properties Odor and Color: Terpenoids often have distinctive odors and colors. Observation of these properties can provide preliminary information. 2. Thin-Layer Chromatography (TLC) Principle: … Read more

Isolation of terpenoids

The isolation of terpenoids from natural sources involves several steps to extract, separate, and purify these compounds. Here’s a general outline of the process: 1. Collection of Plant Material Terpenoids are commonly found in plants. The first step involves collecting plant material known to contain the desired terpenoids. 2. Extraction The plant material is subjected … Read more

Terpenoids – Occurrence, Distribution

Terpenoids, also known as isoprenoids, constitute a diverse and extensive class of natural compounds derived from isoprene units consisting of five carbon atoms each. These organic compounds are essential in various living organisms, including plants, microorganisms, and animals. Terpenoids are characterized by their structural diversity, with numerous variations resulting from the arrangement of isoprene building … Read more

Therapeutic activity and pharmaceutical applications of alkaloids

Alkaloids, a diverse group of naturally occurring organic compounds, exhibit various therapeutic activities. Many alkaloids have been harnessed for their pharmacological effects and find applications in various pharmaceutical formulations. Here’s a detailed note on the therapeutic activity and pharmaceutical applications of alkaloids: 1. Analgesic and Antinociceptive Activity    – Examples: Morphine, Codeine, Papaverine.    – … Read more